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Read our frequently asked questions here, along with tips on how to make the most out of our services

What do I need to consider if providing samples/tissue as FFPE blocks or slides?

Multi-plex Immunofluorescence (IF) Imaging RNAScope Single and Double Antibody Tissue Staining (Chromogenic IHC)

 

  • Old tissue is often poorer quality than newer tissue. We recommend re-embedding in fresh wax prior to use.
  • Tissue is better preserved in the block than on the slide, for example, historically cut slides often give poorer staining. We recommend sectioning close to the staining date.
  • Optimal formalin fixation results in better IHC or multiplex staining.
  • There should only be one tissue section per slide.
  • Place tissue as centrally as possible on the slide.
  • If intending to overlay images for analysis, tissue placement and orientation must be as similar as possible across each slide.
  • Thick tissue sections give poorer quality staining. 5-micron sections are recommended.

Please see the FAQ ‘What factors affect the quality and consistency of multiplexed images?’ for further multiplex related considerations.