Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

We will optimise a protocol to get your antibody staining looking the best it can

In immunohistochemistry there are several factors which can affect the quality of staining, such as the antigen retrieval method, antibody concentration and antibody incubation time. We optimise each of these conditions so that each antibody produces IHC staining on the tissue of interest with an optimal specific staining to background ratio.

Appearance of histopathological scans

Below is a list of antibodies that the lab have previously developed protocols for. NB: Not all antibodies are immediately available and may need to be ordered in on request. Frequently used antibodies are shown with an (*).

Please select how you wish to view this list:

 

Antibody  Tissues Target Description
AID Human Only Somatic hypermutation, gene conversion, and class-switch recombination of Ig genes
Alpha smooth muscle Actin* Human and Mouse Cytoskeleton marker: Muscle cells
Bcl-6 Human Only Activated and proliferating B cells in germinal centres 
CAIX* Human Only Hypoxia marker   
Caspase3 Human Only Apoptosis marker
CD103 Human Only CD103 is expressed widely on intraepithelial lymphocyte (IEL) T cells (both αβ T cells and γδ T cells) and on some peripheral regulatory T cells (Tregs).It has also been reported on lamina propria T cells. A subset of dendritic cells in the gut mucosa and mesenteric lymph nodes, known as CD103 dendritic cells, also expresses this marker.
CD138 Human Only Plasma cells
CD146* Human and Mouse Endothelial (cell lineage) marker
CD16 Human Only CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, and macrophages
CD19* Human Only B cells and follicular dendritic cells
CD2 Human Only CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor.
CD20* Human and Mouse B cells
CD206 Human Only The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages, immature dendritic cells and liver sinusoidal endothelial cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes.
CD21 Human Only Complement receptor type 2 (CR2), also known as complement C3d receptor, Epstein-Barr virus receptor, and CD21 (cluster of differentiation 21), B cells express CR2 receptors on their surfaces, allowing the complement system to play a role in B-cell activation and maturation
CD27 Human Only Memory B cells, some plasma cells, naïve and memory T cells and NK cells.  A useful marker to distinguish certain T and B cell subets from each other.
CD3* Human Only T-cells
CD30 Human and Mouse Reed-Sternberg cells
CD31* Human and Mouse Vascular marker: Endothelial cells
CD32b Human Only Human CD32B (FcγRIIB), the low-affinity inhibitory receptor for IgG, is the predominant Fc receptor (FcR) present on B cells
CD34* Human and Mouse Vascular marker: Endothelial cells
CD35 Human Only CD35 (cluster of differentiation 35) is a protein that in humans is encoded by the CR1 gene, The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, hyalocytes, and splenic follicular dendritic cells
CD38 Human Only CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B lymphocytes and natural killer cells. CD38 also functions in cell adhesion, signal transduction and calcium signaling.
CD4* Human and Mouse T-Helper cells, monocyte/macrophages, granulocytes, thymocytes and Langerhans' cells
CD45 Human Only Protein tyrosine phosphatase, receptor type C. All differentiated hematopoietic cells (except erythrocytes and plasma cells).
CD5 Human Only T -cells
CD56 (NCAM) Human and Mouse Neurons, astrocytes, Schwann cells, NK cells and a subset of activated T cells
CD68* Human and Mouse Macrophages, monocyes, neutrophils, basophils and large lymphocytes, stimulated T cells and NK cells
CD8* Human and Mouse Cytotoxic T-cells
Collagen 1 Mouse
CXCL14 Human and Mouse  (C-X-C motif chemokine ligand 14) Chemokine secreted by cancer associated fibroblasts (CAFs), Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes
CXCR5 Human Only B-cells. Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC).
Cytokeratin 5 Human Only Identifying basal cells or myoepithelial cells in the breast and prostate
Cytokeratin 7 Human Only Cytoskeleton: Simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. 
Desmin Human and Mouse Cytoskeleton: Muscle cells (cardiac, skeletal and smooth)
E-Cadherin* Human and Mouse Epithelial marker (excluding neuronal epithelial): Intracellular junction marker
ENO1 Human Only (Enolase 1) Glycolysis. Cell surface receptor for plasminogen. Ubiquitously expressed in adult human tissues.
EpCAM Mouse Only (Epithelial Cell Adhesion Molecule) Epithelial cell membranes (except mesodermal or neural cell membranes). Undifferentiated embryonic stem cells. Adenocarcinoma.
ER Human Only (Estrogen receptor) Nuclear hormone receptor
FOXP3* Human and Mouse T regulatory cells
GFAP Human Only Astrocyte and neural stem cell marker
Gremlin Mouse Only Cytokine highly expressed in small intestine, fetal brain and colon. Expression is restricted to intestinal subepithelial myofibroblasts (ISEMFs) at the crypt base. In subjects with HMPS1 it is also expressed  at very high levels in epithelial cells (predominantly colonocytes), with expression extending most of the way up the sides of the crypt. 
H3K36me3 Human Only Tri-methylation at the 36th lysine residue of the histone H3 protein associated with gene bodies and maintenance of genomic stability.
Hexon (Adenovirus) Human Only Major capsid protein (all 41 Adenovirus serotypes)
HR23B Human Only Nucleotide excision repair
Hypoxyprobe-1MAb1Anti-pimonidazole Human Only Hypoxia marker (pimonidazole administered)
Ki-67* Human Only Proliferating cells
Laminin Mouse Only B1 nuclear envelope marker
LAMP3 Human Only Dendritic cells
LARP1 Human Only RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability
Ly6g Mouse Only Predominantly neutrophils but also a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
MAP2 Human Only (Microtubule associated protein 2) Neurons 
MECA-79 (PNAd) Human Only Vascular marker: High Endothelial Venule (HEV)
Melan A Human Only Melanocyte differentiation marker 
MPO Human Only Monocytes, myeloid dentritic cells, neutrophils. Part of the host defense system of polymorphonuclear leukocytes.
Pan-Cytokeratin* Human Only Cytoskeleton: Epithelial cells
PD-L1* Human Only Important checkpoint marker: Macrophages, T cells, B cells, NK cells and DCs
PD1* Human Only Important checkpoint marker: T cells and pro-B cells
Periostin Human and Mouse Cell adhesion marker: Heparin
Podoplanin Human and Mouse Lymphatic endothelial marker
PR Human Only (Progesterone receptor) Hormone receptor
Semaphorin 3A Human and Mouse  Axon guidance regulation marker: Meningeal fibroblasts
SOX6 Mouse Only Marker of developmental processes: CNS, cardiac and skeletal muscle cells
SQSTM-p62 Human Only (Sequestosome-1 is a protein is encoded by SQSTM1 gene also known as the ubiquitin-binding protein p62) autophagosome cargo protein, autophagosome marker: ubiquitously expressed in tissue.
TIA1 Human Only TIA1 cytotoxic granule associated RNA binding protein that possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells.
TNFR1 Human Only (Tumor necrosis factor receptor 1): cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation:  mainly secreted by macrophages
VEGF Human Only (Vascular endothelial growth factor) Angiogenesis, tumour angiogenesis and vasculogenesis marker
VEGFR2 Human Only (Vascular endothelial growth factor receptor 2) accounts for most of the mitogenic and chemotactic effects of VEGF: Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

Immunology

Antibody  Tissues Target Description
AID Human Only Somatic hypermutation, gene conversion, and class-switch recombination of Ig genes
Bcl-6 Human Only Activated and proliferating B cells in germinal centres 
Caspase3 Human Only Apoptosis marker
CD103 Human Only CD103 is expressed widely on intraepithelial lymphocyte (IEL) T cells (both αβ T cells and γδ T cells) and on some peripheral regulatory T cells (Tregs).It has also been reported on lamina propria T cells. A subset of dendritic cells in the gut mucosa and mesenteric lymph nodes, known as CD103 dendritic cells, also expresses this marker.
CD138 Human Only Plasma cells
CD16 Human Only CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, and macrophages
CD19 Human Only B cells and follicular dendritic cells
CD2 Human Only CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor.
CD20 Human and Mouse B cells
CD206 Human Only The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages, immature dendritic cells and liver sinusoidal endothelial cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes.
CD21 Human Only Complement receptor type 2 (CR2), also known as complement C3d receptor, Epstein-Barr virus receptor, and CD21 (cluster of differentiation 21), B cells express CR2 receptors on their surfaces, allowing the complement system to play a role in B-cell activation and maturation
CD27 Human Only Memory B cells, some plasma cells, naïve and memory T cells and NK cells.  A useful marker to distinguish certain T and B cell subets from each other.
CD3 Human Only T-cells
CD30 Human and Mouse Reed-Sternberg cells
CD32b Human Only Human CD32B (FcγRIIB), the low-affinity inhibitory receptor for IgG, is the predominant Fc receptor (FcR) present on B cells
CD35 Human Only CD35 (cluster of differentiation 35) is a protein that in humans is encoded by the CR1 gene, The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, hyalocytes, and splenic follicular dendritic cells
CD4 Human and Mouse T-Helper cells, monocyte/macrophages, granulocytes, thymocytes and Langerhans' cells
CD45 Human Only Protein tyrosine phosphatase, receptor type C. All differentiated hematopoietic cells (except erythrocytes and plasma cells).
CD5 Human Only T -cells
CD56 (NCAM) Human and Mouse Neurons, astrocytes, Schwann cells, NK cells and a subset of activated T cells
CD68 Human and Mouse Macrophages, monocyes, neutrophils, basophils and large lymphocytes, stimulated T cells and NK cells
CD8 Human and Mouse Cytotoxic T-cells
CXCL14 Human and Mouse  (C-X-C motif chemokine ligand 14) Chemokine secreted by cancer associated fibroblasts (CAFs), Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes
CXCR5 Human Only B-cells. Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC).
FOXP3 Human and Mouse T regulatory cells
LAMP3 Human Only Dendritic cells
Ly6g Mouse Only Predominantly neutrophils but also a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
MECA-79 (PNAd) Human Only Vascular marker: High Endothelial Venule (HEV)
MPO Human Only Monocytes, myeloid dentritic cells, neutrophils. Part of the host defense system of polymorphonuclear leukocytes.
PD-L1 Human Only Important checkpoint marker: Macrophages, T cells, B cells, NK cells and DCs
PD1 Human Only Important checkpoint marker: T cells and pro-B cells
Podoplanin Human and Mouse Lymphatic endothelial marker
TIA1 Human Only TIA1 cytotoxic granule associated RNA binding protein that possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells.
TNFR1 Human Only (Tumor necrosis factor receptor 1): cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation:  mainly secreted by macrophages

 

Metabolic

Antibody Tissues Target Description
CAIX Human Only Hypoxia marker   
Hypoxyprobe-1MAb1Anti-pimonidazole Human Only Hypoxia marker (pimonidazole administered)

 

Neurology

Antibody Tissues Target Description
GFAP Human Only Astrocyte and neural stem cell marker
MAP2 Human Only (Microtubule associated protein 2) Neurons 

 

Stroma 

Antibody Tissues Target Description
Alpha smooth muscle Actin Human and Mouse Cytoskeleton marker: Muscle cells
CD146 Human and Mouse Endothelial (cell lineage) marker
CD31 Human and Mouse Vascular marker: Endothelial cells
CD34 Human and Mouse Vascular marker: Endothelial cells
Gremlin Mouse Only Cytokine highly expressed in small intestine, fetal brain and colon. Expression is restricted to intestinal subepithelial myofibroblasts (ISEMFs) at the crypt base. In subjects with HMPS1 it is also expressed  at very high levels in epithelial cells (predominantly colonocytes), with expression extending most of the way up the sides of the crypt. 
Laminin Mouse Only B1 nuclear envelope marker
Periostin Human and Mouse Cell adhesion marker: Heparin
VEGF Human Only (Vascular endothelial growth factor) Angiogenesis, tumour angiogenesis and vasculogenesis marker
VEGFR2 Human Only (Vascular endothelial growth factor receptor 2) accounts for most of the mitogenic and chemotactic effects of VEGF: Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

Epithelium 

Antibody Tissues Target Description
Cytokeratin 5 Human Only Identifying basal cells or myoepithelial cells in the breast and prostate
Cytokeratin 7 Human Only Cytoskeleton: Simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. 
E-Cadherin Human and Mouse Epithelial marker (excluding neuronal epithelial): Intracellular junction marker
EpCAM Mouse Only (Epithelial Cell Adhesion Molecule) Epithelial cell membranes (except mesodermal or neural cell membranes). Undifferentiated embryonic stem cells. Adenocarcinoma.
ER Human Only (Estrogen receptor) Nuclear hormone receptor
LARP1 Human Only RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability
Pan-Cytokeratin Human Only Cytoskeleton: Epithelial cells
PR Human Only (Progesterone receptor) Hormone receptor
Antibody Target Description
Alpha smooth muscle Actin Cytoskeleton marker: Muscle cells
CD146 Endothelial (cell lineage) marker
CD20 B cells
CD30 Reed-Sternberg cells
CD31 Vascular marker: Endothelial cells
CD34 Vascular marker: Endothelial cells
CD4 T-Helper cells, monocyte/macrophages, granulocytes, thymocytes and Langerhans' cells
CD56 (NCAM) Neurons, astrocytes, Schwann cells, NK cells and a subset of activated T cells
CD68 Macrophages, monocyes, neutrophils, basophils and large lymphocytes, stimulated T cells and NK cells
CD8 Cytotoxic T-cells
Collagen 1
CXCL14  (C-X-C motif chemokine ligand 14) Chemokine secreted by cancer associated fibroblasts (CAFs), Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes
Desmin Cytoskeleton: Muscle cells (cardiac, skeletal and smooth)
E-Cadherin Epithelial marker (excluding neuronal epithelial): Intracellular junction marker
EpCAM (Epithelial Cell Adhesion Molecule) Epithelial cell membranes (except mesodermal or neural cell membranes). Undifferentiated embryonic stem cells. Adenocarcinoma.
FOXP3 T regulatory cells
Gremlin Cytokine highly expressed in small intestine, fetal brain and colon. Expression is restricted to intestinal subepithelial myofibroblasts (ISEMFs) at the crypt base. In subjects with HMPS1 it is also expressed  at very high levels in epithelial cells (predominantly colonocytes), with expression extending most of the way up the sides of the crypt. 
Laminin B1 nuclear envelope marker
Ly6g Predominantly neutrophils but also a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
Periostin Cell adhesion marker: Heparin
Podoplanin Lymphatic endothelial marker
Semaphorin 3A  Axon guidance regulation marker: Meningeal fibroblasts
SOX6 Marker of developmental processes: CNS, cardiac and skeletal muscle cells
Antibody Target Description
AID Somatic hypermutation, gene conversion, and class-switch recombination of Ig genes
Alpha smooth muscle Actin Cytoskeleton marker: Muscle cells
Bcl-6 Activated and proliferating B cells in germinal centres 
CAIX Hypoxia marker   
Caspase3 Apoptosis marker
CD103 CD103 is expressed widely on intraepithelial lymphocyte (IEL) T cells (both αβ T cells and γδ T cells) and on some peripheral regulatory T cells (Tregs).It has also been reported on lamina propria T cells. A subset of dendritic cells in the gut mucosa and mesenteric lymph nodes, known as CD103 dendritic cells, also expresses this marker.
CD138 Plasma cells
CD146 Endothelial (cell lineage) marker
CD16 CD16, also known as FcγRIII, is a cluster of differentiation molecule found on the surface of natural killer cells, neutrophils, monocytes, and macrophages
CD19 B cells and follicular dendritic cells
CD2 CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor.
CD20 B cells
CD206 The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages, immature dendritic cells and liver sinusoidal endothelial cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes.
CD21 Complement receptor type 2 (CR2), also known as complement C3d receptor, Epstein-Barr virus receptor, and CD21 (cluster of differentiation 21), B cells express CR2 receptors on their surfaces, allowing the complement system to play a role in B-cell activation and maturation
CD27 Memory B cells, some plasma cells, naïve and memory T cells and NK cells.  A useful marker to distinguish certain T and B cell subets from each other.
CD3 T-cells
CD30 Reed-Sternberg cells
CD31 Vascular marker: Endothelial cells
CD32b Human CD32B (FcγRIIB), the low-affinity inhibitory receptor for IgG, is the predominant Fc receptor (FcR) present on B cells
CD34 Vascular marker: Endothelial cells
CD35 CD35 (cluster of differentiation 35) is a protein that in humans is encoded by the CR1 gene, The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, hyalocytes, and splenic follicular dendritic cells
CD38 CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B lymphocytes and natural killer cells. CD38 also functions in cell adhesion, signal transduction and calcium signaling.
CD4 T-Helper cells, monocyte/macrophages, granulocytes, thymocytes and Langerhans' cells
CD45 Protein tyrosine phosphatase, receptor type C. All differentiated hematopoietic cells (except erythrocytes and plasma cells).
CD5 T -cells
CD56 (NCAM) Neurons, astrocytes, Schwann cells, NK cells and a subset of activated T cells
CD68 Macrophages, monocyes, neutrophils, basophils and large lymphocytes, stimulated T cells and NK cells
CD8 Cytotoxic T-cells
CXCL14  (C-X-C motif chemokine ligand 14) Chemokine secreted by cancer associated fibroblasts (CAFs), Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes
CXCR5 B-cells. Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC).
Cytokeratin 5 Identifying basal cells or myoepithelial cells in the breast and prostate
Cytokeratin 7 Cytoskeleton: Simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. 
Desmin Cytoskeleton: Muscle cells (cardiac, skeletal and smooth)
E-Cadherin Epithelial marker (excluding neuronal epithelial): Intracellular junction marker
ENO1 (Enolase 1) Glycolysis. Cell surface receptor for plasminogen. Ubiquitously expressed in adult human tissues.
ER (Estrogen receptor) Nuclear hormone receptor
FOXP3 T regulatory cells
GFAP Astrocyte and neural stem cell marker
H3K36me3 Tri-methylation at the 36th lysine residue of the histone H3 protein associated with gene bodies and maintenance of genomic stability.
Hexon (Adenovirus) Major capsid protein (all 41 Adenovirus serotypes)
HR23B Nucleotide excision repair
Hypoxyprobe-1MAb1Anti-pimonidazole Hypoxia marker (pimonidazole administered)
Ki-67 Proliferating cells
LAMP3 Dendritic cells
LARP1 RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability
MAP2 (Microtubule associated protein 2) Neurons 
MECA-79 (PNAd) Vascular marker: High Endothelial Venule (HEV)
Melan A Melanocyte differentiation marker 
MPO Monocytes, myeloid dentritic cells, neutrophils. Part of the host defense system of polymorphonuclear leukocytes.
Pan-Cytokeratin Cytoskeleton: Epithelial cells
PD-L1 Important checkpoint marker: Macrophages, T cells, B cells, NK cells and DCs
PD1 Important checkpoint marker: T cells and pro-B cells
Periostin Cell adhesion marker: Heparin
Podoplanin Lymphatic endothelial marker
PR (Progesterone receptor) Hormone receptor
Semaphorin 3A  Axon guidance regulation marker: Meningeal fibroblasts
SQSTM-p62 (Sequestosome-1 is a protein is encoded by SQSTM1 gene also known as the ubiquitin-binding protein p62) autophagosome cargo protein, autophagosome marker: ubiquitously expressed in tissue.
TIA1 TIA1 cytotoxic granule associated RNA binding protein that possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells.
TNFR1 (Tumor necrosis factor receptor 1): cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation:  mainly secreted by macrophages
VEGF (Vascular endothelial growth factor) Angiogenesis, tumour angiogenesis and vasculogenesis marker
VEGFR2 (Vascular endothelial growth factor receptor 2) accounts for most of the mitogenic and chemotactic effects of VEGF: Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma