Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.


  • Old tissue is often poorer quality than newer tissue. We recommend re-embedding in fresh wax prior to use.
  • Tissue is better preserved in the block than on the slide, for example, historically cut slides often give poorer staining. We recommend sectioning close to the staining date.
  • Optimal formalin fixation results in better IHC or multiplex staining.
  • There should only be one tissue section per slide.
  • Place tissue as centrally as possible on the slide.
  • If intending to overlay images for analysis, tissue placement and orientation must be as similar as possible across each slide.
  • Thick tissue sections give poorer quality staining. 5-micron sections are recommended.

Please see the FAQ ‘What factors affect the quality and consistency of multiplexed images?’ for further multiplex related considerations.