What do I need to consider if providing samples/tissue as FFPE blocks or slides?
- Old tissue is often poorer quality than newer tissue. We recommend re-embedding in fresh wax prior to use.
- Tissue is better preserved in the block than on the slide, for example, historically cut slides often give poorer staining. We recommend sectioning close to the staining date.
- Optimal formalin fixation results in better IHC or multiplex staining.
- There should only be one tissue section per slide.
- Place tissue as centrally as possible on the slide.
- If intending to overlay images for analysis, tissue placement and orientation must be as similar as possible across each slide.
- Thick tissue sections give poorer quality staining. 5-micron sections are recommended.
Please see the FAQ ‘What factors affect the quality and consistency of multiplexed images?’ for further multiplex related considerations.