What factors affect the quality and consistency of brightfield images?
- Staining is dependent on antibody quality and epitope expression levels.
- Endogenous pigment can interfere with analysis in bright field imaging (melanin, carbon, tattoo, haemosiderin). We recommend choosing an appropriate coloured chromogen.
- Targets where there is a validated clinical grade antibody will show better results.
- Cell localised targets, rather than connective tissue or stroma localised tissue, will often give better analysis results.