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Differences in metabolic processes in normal and cancer cells (e.g. hypoxia – low oxygen) present opportunities for targeted therapies, but can also interfere with standard treatments such as radiotherapy. These metabolic differences are driven by local, genetic and environmental factors and other co-morbidities such as obesity. It is therefore important to understand hypoxia, signalling, nucleotide metabolism, obesity and drug associated changes to metabolism and how they affect cell behaviour.