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Mechanistic and multiscale modelling allow us to simulate biological process and interactions (such as hypoxia, immune cell infiltration, DNA dynamics), as well as functions that vary within space and time (tumour evolution, cell signalling). In doing so, we can make predictions based on hypothetical mechanisms in the context of experimental observations, and novel hypotheses can be derived for further testing. In addition, novel methods for analysing biological data are being generated (such as topological data analysis), which can be tailored to the noisy and often incomplete datasets generated during laboratory analysis and clinical research.