What factors affect the quality and consistency of multiplexed images?
- Large tissue sections and TMAs show more variation than smaller sections. Sections no larger than 2cmx2cm are recommended.
- We recommend the use of TOMO slides which preserve tissue substantially better than conventional coated slides.
- Epitopes that share the same sub-cellular localisation (e.g.in the same receptor complex) may be prone to steric hindrance. Careful selection of panels is therefore recommended.
Please see the FAQ ‘What factors affect the quality and consistency of brightfield images?’ for further considerations.