Immunology-oncology & Biochemistry

How we can help you

We combine established expertise with newly developed capabilities to support both pre-clinical and clinical research, from primary cell processing and immune assays through to regulated workflows, data management and project delivery.

Laboratory Platforms

IOB operates a range of specialist laboratory platforms and facilities supporting immune monitoring, assay development and primary cell processing.

• ^{Flow and spectral cytometry: Aurora and BD Fortessa cytometry platforms supporting conventional and spectral flow cytometry.}
• ^{ELISpot and FluoroSpot: Platforms for high-throughput immune-response assays, supported by dedicated plate storage, workspace and sample-tracking equipment.}
• ^{Luminex and ELISA: Facilities for cytokine and biomarker analysis and assay development, including automated plate washing, dedicated workbench space and sample-tracking facilities.}
• ^{Class II tissue culture: Dedicated tissue-culture facilities equipped with biological safety cabinets, incubators with temperature and CO₂ monitoring, centrifuges, cold storage and standard laboratory equipment.}
• ^{Sample and reagent management: Dedicated reception and tracking facilities, with controlled waste streams supporting laboratory workflows.}

Scientific Capabilities

• ^{Flow cytometry: Bulk-cell sorting, pHLA tetramer/dextramer staining for TCR identification, and small-panel immunophenotyping across multiple cytometry platforms.}
• ^{Spectral flow cytometry: Large-panel immunophenotyping, including intracellular cytokine staining (ICS).}
• ^{Cytokine analysis: ELISA, Luminex and MSD platforms for cytokine-release assessment.}
• ^{Primary cell isolation and culture: PBMC isolation, cryopreservation and thawing; immune-cell subset isolation; generation of monocyte-derived dendritic cells (moDCs) and activated primary B cells; and cell-line culture.}
• ^{Cell biology: T-cell and PBMC stimulation using peptide pools, alongside extended culture and expansion of T cells expressing TCRs of interest.}
• ^{Peptide handling and immune-response assays: High-throughput IFNγ ELISpot, and multiplex FluoroSpot for the detection of up to four secreted markers.}
• ^{Quality and regulatory expertise: Experience working within GLP, GMP and HTA frameworks, including SOP development, reagent validation, instrument IQ/OQ/PQ, assay validation and verification, EQA, reporting and auditing.}
• ^{Clinical and sample support: Qualified phlebotomy expertise and donor database coordination, supporting integration between laboratory and clinical activities.}
• ^{Laboratory data and tracking: LIMS and ELN superuser expertise covering reagent, sample and experiment tracking, user training and basic SQL scripting.}